LOCUS       40924_31935       12745 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pMpGWB431 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12745)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T.
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha
  JOURNAL   PLoS ONE 10 (9), E0138876 (2015)
  PUBMED    26406247
REFERENCE   2  (bases 1 to 12745)
  AUTHORS   Nishihama R, Ishizaki K, Kohchi T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, 
            Graduate School of Biostudies; Kitashirakawa-oiwake-cho, Sakyo-ku, 
            Kyoto, Kyoto 606-8502, Japan URL :http://www.lif.kyoto-u.ac.jp/e/
REFERENCE   3  (bases 1 to 12745)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12745)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2015"; volume: "10"; issue: "9"; pages: "E0138876"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, Graduate 
            School of Biostudies"; volume: " Kitashirakawa-oiwake-cho, Sakyo-ku,
            Kyoto, Kyoto 606-8502, Japan URL :http"; pages: 
            "//www.lif.kyoto-u.ac.jp/e"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12745
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    323..447
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        484..514
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             568..1224
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1569..1871
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1915..2039)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             3522..3530
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             3676..3795
                     /codon_start=1
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     terminator      3820..4072
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4106..4122)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4130..4146
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4154..4184)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4199..4220)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     polyA_signal    complement(4305..4479)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4539..5327)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(5396..6072)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    complement(6267..6291)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6812..7600
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7849..8437
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8623..8763)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9107..9301)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9370..10440)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10872..11498)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      361 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      421 taaaacacaa catatccagt cactatggcg gcccctcgag gggccgcatt aggcacccca
      481 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccggcg
      541 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      601 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      661 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      721 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      781 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      841 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
      901 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
      961 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     1021 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1081 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1141 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1201 tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg gcttactaaa agccagataa
     1261 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     1321 cgaagtatgt caaaaagagg tgtgctatga agcagcgtat tacagtgaca gttgacagcg
     1381 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     1441 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     1501 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacaggga
     1561 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     1621 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     1681 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     1741 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     1801 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     1861 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
     1921 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     1981 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
     2041 gataacagca tggagagaga gaagaacgtg gtgtacggcc ccgagcccaa gcaccctctg
     2101 ggcaacttca ccgccggcga gatgctgtac aacgctctgc acaagcactc ccacatcccc
     2161 caggccatcc tggacgtgat gggcaacgag tccctttcct accaggagtt cttcgacact
     2221 actgtgaagc tgggccagag cctccagaac tgtggctaca agatgaacga tgtcgtgtcg
     2281 atctgtgcag agaacaacaa gagattcttc atccccatca tctccgcctg gtacatcggc
     2341 atggtggtgg cccctgtgaa cgaggactat atcccagacg agctgtgtaa agtgaccggc
     2401 atctccaagc cgatcctggt cttcaccact aggaagatcc tgcctaaggt tttggaggtt
     2461 aaagacagaa ccaactacat aaagaggatc atcatactgg actctgaaga gaacctgctg
     2521 ggctgcgaga gcctgcacaa cttcatgtcc aggtactccg acaacaacct ccaaacattc
     2581 aagcctctgc actacgaccc tgtggaccag gtagccgcca tcctgtgctc ctccggcaca
     2641 accggcctgc ctaaaggcgt gatgcagacc cacaggaaca tctgtgtgag actcacacac
     2701 gcatctgacc ccagagtggg tacacaactc atccccggcg tatccgtgct ggcctacctg
     2761 ccattcttcc acgccttcgg cttcagtatc aacctgggct atttcatggt gggcctgaga
     2821 gtggtgatgc tccgaaggtt taaccaggag gtgttcctga aggccatcca ggactacgag
     2881 gtgaggagcg tgatcaacgt tccctccaca atcctgttcc tgtccaagag ccctctggtg
     2941 gacaagtacg acctatccac cctggcggag ctgtgctgtg gagccgctcc tctggcgaag
     3001 gaggtggccg agatcgccgt gaagaggctg aacctgccag ggatacggtg tggctacggt
     3061 ctaacagagt ctacctccgc caacatccat actctgcaca acgagttcaa gtccggctcc
     3121 ctgggcaagg tgacacctta catggccgcc aagatcatcg acaggaacac cggcgaggcc
     3181 ctgggtccaa accaggtggg cgagctgtgc atctggggac ctatggtaac aaaaggctat
     3241 gtgaacaacc cacaggctac taaggaggcc atcgacgacg acggctggct gcactctggc
     3301 gacttcggct actacgacga ggacgagtat ttctacatcg tggaccggta caaggagctg
     3361 atcaaataca agggctatca ggtcgcccct gtggagctgg aggagatcct ccttcagcac
     3421 ccaggcatca gggacgtggc cgtcgtgggt atccctgaca tcgaggccgg cgagctgcca
     3481 gccggcttcg tggtgaagca gcccggcgcc caactcaccg ctaaggaggt gtacgacttc
     3541 ctggcccaga gggtgtctca ctccaagtac ctgaggggcg gcgtaaggtt cgtggactct
     3601 atccccagga acgtgacagg caagattagt cgaaaagagc tgagggaggc cctgatggag
     3661 aaggcttcta agcttagcca tggcttcccg ccggaggtgg aggagcaggc tgctggcacg
     3721 ctgcccatgt cttgtgccca ggagagcggg atggaccgtc accctgcagc ctgtgcttct
     3781 gctaggatca atgtgtaggc ttagagctcg aatttccccg atcgttcaaa catttggcaa
     3841 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     3901 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     3961 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     4021 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg
     4081 gttccggaac caattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     4141 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     4201 gagtgagcta actcacatta ggctgaatta ggcgcgccta tttctgaatt caacacattg
     4261 cggacgtttt taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta
     4321 ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat
     4381 acatactaag ggtttcttat atgctcaaca catgagcgaa accctatagg aaccctaatt
     4441 cccttatctg ggaactactc acacattatt atggagaaac tcgagcttgt cgatcgactc
     4501 tagctagagg atcgatccga accccagagt cccgctcaga agaactcgtc aagaaggcga
     4561 tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca
     4621 gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag
     4681 cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc
     4741 atgatattcg gcaagcaggc atcgccatgt gtcacgacga gatcctcgcc gtcgggcatg
     4801 cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga
     4861 tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc
     4921 gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca
     4981 gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc
     5041 acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg
     5101 caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg gagttcattc
     5161 agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg
     5221 aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc
     5281 tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatccc catggtcgat
     5341 cgacagatct gcgaaagctc gagagagata gatttgtaga gagagactgg tgatttcagc
     5401 gtgtcctctc caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt
     5461 gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag
     5521 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg
     5581 gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt
     5641 tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat
     5701 ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt
     5761 cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt
     5821 tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc
     5881 ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc
     5941 tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg
     6001 atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt
     6061 tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag
     6121 acgagagtgt cgtgctccac catgttggca agctgctcta gccaatacgc aaaccgaatt
     6181 cagaaataaa ttcagcctaa ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg
     6241 ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc
     6301 caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca
     6361 gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg
     6421 atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga
     6481 gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa ttcgggcacg
     6541 aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc
     6601 acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt
     6661 catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca
     6721 agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca
     6781 gggcagtcgc cctaaaacaa agttaaacat catgggggaa gcggtgatcg ccgaagtatc
     6841 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc
     6901 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt
     6961 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct
     7021 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat
     7081 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg
     7141 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga
     7201 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc
     7261 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt
     7321 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt
     7381 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga
     7441 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc
     7501 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
     7561 ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatgtctag ctagaaattc
     7621 gttcaagccg acgccgcttc gcggcgcggc ttaactcaag cgttagatgc actaagcaca
     7681 taattgctca cagccaaact atcaggtcaa gtctgctttt attattttta agcgtgcata
     7741 ataagcccta cacaaattgg gagatatatc atgcatgacc aaaatccctt aacgtgagtt
     7801 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     7861 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     7921 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     7981 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8041 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8101 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8161 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     8221 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     8281 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     8341 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     8401 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     8461 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     8521 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     8581 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct
     8641 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
     8701 tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct
     8761 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     8821 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     8881 tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga
     8941 cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt aggccagcca tttttgagcg
     9001 gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag
     9061 ggtaggcgct ttttgcagct cttcggctgt gcgctggcca gacagttatg cacaggccag
     9121 gcgggtttta agagttttaa taagttttaa agagttttag gcggaaaaat cgcctttttt
     9181 ctcttttata tcagtcactt acatgtgtga ccggttccca atgtacggct ttgggttccc
     9241 aatgtacggg ttccggttcc caatgtacgg ctttgggttc ccaatgtacg tgctatccac
     9301 aggaaagaga ccttttcgac ctttttcccc tgctagggca atttgcccta gcatctgctc
     9361 cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag
     9421 gatcgggcca gcctgccccg cctcctcctt caaatcgtac tccggcaggt catttgaccc
     9481 gatcagcttg cgcacggtga aacagaactt cttgaactct ccggcgctgc cactgcgttc
     9541 gtagatcgtc ttgaacaacc atctggcttc tgccttgcct gcggcgcggc gtgccaggcg
     9601 gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac
     9661 gtccgggttc ttgccttctg tgatctcgcg gtacatccaa tcagctagct cgatctcgat
     9721 gtactccggc cgcccggttt cgctctttac gatcttgtag cggctaatca aggcttcacc
     9781 ctcggatacc gtcaccaggc ggccgttctt ggccttcttc gtacgctgca tggcaacgtg
     9841 cgtggtgttt aaccgaatgc aggtttctac caggtcgtct ttctgctttc cgccatcggc
     9901 tcgccggcag aacttgagta cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc
     9961 tcccttccct tcccggtatc ggttcatgga ttcggttaga tgggaaaccg ccatcagtac
    10021 caggtcgtaa tcccacacac tggccatgcc ggccggccct gcggaaacct ctacgtgccc
    10081 gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa
    10141 aacggccacg tccatgatgc tgcgactatc gcgggtgccc acgtcataga gcatcggaac
    10201 gaaaaaatct ggttgctcgt cgcccttggg cggcttccta atcgacggcg caccggctgc
    10261 cggcggttgc cgggattctt tgcggattcg atcagcggcc gcttgccacg attcaccggg
    10321 gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc gcggccttca acttctccac
    10381 caggtcatca cccagcgccg cgccgatttg taccgggccg gatggtttgc gaccgctcac
    10441 gccgattcct cgggcttggg ggttccagtg ccattgcagg gccggcagac aacccagccg
    10501 cttacgcctg gccaaccgcc cgttcctcca cacatggggc attccacggc gtcggtgcct
    10561 ggttgttctt gattttccat gccgcctcct ttagccgcta aaattcatct actcatttat
    10621 tcatttgctc atttactctg gtagctgcgc gatgtattca gatagcagct cggtaatggt
    10681 cttgccttgg cgtaccgcgt acatcttcag cttggtgtga tcctccgccg gcaactgaaa
    10741 gttgacccgc ttcatggctg gcgtgtctgc caggctggcc aacgttgcag ccttgctgct
    10801 gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt ttgctcattt tctctttacc
    10861 tcattaactc aaatgagttt tgatttaatt tcagcggcca gcgcctggac ctcgcgggca
    10921 gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag
    10981 ctcacgcgct gcgtgatacg ggactcaaga atgggcagct cgtacccggc cagcgcctcg
    11041 gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt
    11101 agccttccat ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc
    11161 catatgtcgt aagggcttgg ctgcaccgga atcagcacga agtcggctgc cttgatcgcg
    11221 gacacagcca agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg
    11281 atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt tagcggttga
    11341 tcttcccgca cggccgccca atcgcgggca ctgccctggg gatcggaatc gactaacaga
    11401 acatcggccc cggcgagttg cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct
    11461 gacccgcctt tctggttaag tacagcgata accttcatgc gttccccttg cgtatttgtt
    11521 tatttactca tcgcatcata tacgcagcga ccgcatgacg caagctgttt tactcaaata
    11581 cacatcacct ttttagacgg cggcgctcgg tttcttcagc ggccaagctg gccggccagg
    11641 ccgccagctt ggcatcagac aaaccggcca ggatttcatg cagccgcacg gttgagacgt
    11701 gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc cgcctcgatc tcttcggtaa
    11761 tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct cttggcgttc
    11821 attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg
    11881 ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt acagggtcga
    11941 gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg cggccttcct ggtcgatcag
    12001 ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg cgggggccaa acttcacgcc
    12061 tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa
    12121 ctcggcaatg ccggcgaaca cggtcaacac catgcggccg gccggcgtgg tggtgtcggc
    12181 ccacggctct gccaggctac gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc
    12241 cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg gtcactgtca caacgtcgcc
    12301 agggcgtagg tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat
    12361 cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa
    12421 gtcctggtcg tcggtgctga cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt
    12481 catggcgtaa tgtctccggt tctagtcgca agtattctac tttatgcgac taaaacacgc
    12541 gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt aggacttgtg
    12601 cgacatgtcg ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca
    12661 gcggaggggt tggatcaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca
    12721 tacaaatgga cgaacggata aacct
//
